Electronic Supplementary Information (ESI) available: Evolution of the absorbance of 1,3-diphenylisobenzofuran (DBF) and curcumin alone at 480 nm in acetonitrile under visible light illumination and emission spectrum of the 4 lamps used during the different incubation times. SeeThe development of new antibacterial coating (against Escherichia coli and Staphylococcus aureus) with the use of a natural dye (curcumin) and epoxidized soybean oil, according to a photochemistry process has been investigated.Curcumin has been used both as a photosensitizer and an antibacterial agent under visible light illumination. The photoinduced coatings show good adherence properties on inox substrate and a high thermal stability by 375°C. Under visible light activation, singlet oxygen ( 1 O2) could be generated from the curcumin derivative-coatings, thus inhibiting by 99% and 95% the growth of Staphylococcus aureus and Escherichia coli, respectively, even after 48h of incubation.
The influence of copper derived TiO surfaces (nCu-nT-TiO) on the death of nosocomial Staphylococcus aureus (Sa) and Escherichia coli (Ec), was investigated. TiO nanotube (nT-TiO) arrays were fabricated by anodic oxidation of pure titanium sheets in fluorhydric solutions, leading to surface nanostructuration and creation of specific reactive sites. Copper nanocubes with a mean size of 20 nm have been synthesized and deposited on the nT-TiO surface by pulsed electrodeposition from a copper sulphate solution. Scanning Electron Microscopy (SEM) reveals that Cu nanocubes are both inserted into the TiO nanotubes and on the nanotube edges. X-ray Photoemission Spectroscopy (XPS) and SEM-EDX confirm the metallic nature of copper nanoparticles, covered with a thin mixed CuO-CuO thin layer. As the adsorption of proteins is one of the early stages of biomaterial surface interactions with body fluids before bacterial colonization, Infrared Spectroscopy (IR) in reflection-absorption mode, SEM and XPS have been used to follow the evolution of nCu-nT-TiO surfaces when exposed to a simulated plasma solution containing Bovine Serum Albumin (BSA). Finally bacterial tests have revealed a high biocide potential of the nCu-nT-TiO surface, which leads to the entire death of SA and EC.
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