ResumenObjetivo. Estudiar cepas asociadas a la comunidad de SARM y productoras de PVL en individuos sanos de la ciudad de Montería. Materiales y métodos. Estudio descriptivo, prospectivo de corte transversal, a partir de un total de 253 muestras obtenidas de hisopados faríngeos en tres comunidades: 91 internos de la cárcel de Montería (19-58 años), 100 estudiantes adultos jóvenes de la Universidad de Córdoba (17-30 años) y 62 niños en edad escolar (4-9 años) del Colegio de la Universidad de Córdoba. Los individuos participantes no estuvieron hospitalizados en los últimos meses, ni recibieron tratamiento antimicrobiano y no presentaron signos y/o síntomas clínicos. Los aislamientos fueron identificados por pruebas convencionales microbiológicas y se determinó la susceptibilidad antimicrobiana para los diferentes antibióticos a través de MicroScan combo 1A (SIEMENS). Para la detección de los genes nuc, mecA y PVL se utilizaron los protocolos de Brakstad et al (5), Oliveira et al (6) y Gerard et al (7) respectivamente. Resultados. De las 253 muestras analizadas, 62 (24,5%) resultaron positivas para S. aureus, de éstas 4 (6,45%) fueron resistentes a meticilina, de las cuales 2 (25%) resultaron PVL positivas; 58 (93,54%) fueron sensibles y 6 (75%) positivas para PVL. Conclusiones. Los resultados obtenidos demuestran la colonización por SARM-AC en individuos sanos, la colonización fue mayor en la población infantil y adultos jóvenes.Palabras clave: Staphylococcus aureus, resistencia, meticilina, PVL, comunidad, Colombia. Abstract PVL positive methicillin-resistant Staphylococcus aureus (MRSAs) strains isolated from healthy individuals in Montería,Córdoba. Objective. To study SARM and PVL producing strains in healthy individuals of Montería city. Materials and methods. This was a descriptive, prospective and transversal study. A total of 253 samples from pharyngeal swabs in three communities were studied: 91 prisoners of the jail of Montería (19-58 years old), 100 young adult students of the University of Cordoba (17-30 years old) and 62 children in school age (4-9 years old) of the primary school of the University of Cordoba. Individuals analyzed had not been hospitalized, had not received any antimicrobial treatment and did not show any clinical signs or symptoms in the last months. The isolates were identified by conventional microbiological tests and the antimicrobial susceptibility was determined for different antibiotics with MicroScan™ kit 1A (SIEMENS). For the detection of the nuc, mecA and PVL genes, we followed the protocols of Brakstad et al., Oliveira et al. and Gerard et al. respectively. Results. Of the 253 analyzed samples, 62 (24.5%) were positive for S. aureus, out of those 4 (6.45%) were resistant to methicillin and 2 (25%) of them were PVL positive; 58 (93.54%) were sensitive to methicillin and 6 (75%) positive for PVL. Conclusions. Results obtained in this study show the colonization by SARM-AC of healthy individuals. Colonization was higher in young adults and children in school-age.
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