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The four serotypes of dengue virus (DENV1-4) are the causal agents of the emerging disease Dengue Fever and its severe forms. DENV is inoculated into human blood through a mosquito bite. Thus, plasma is an important media for DENV dissemination in infected persons and several important interactions should take place for the virus with human plasma proteins that strongly influence or may determine the course of the infection. This dataset contains 239 proteins identified in the elution fractions of human plasma subjected to DE-52 anion exchange chromatography. Data on DENV2 infection of Huh 7.5 cells in presence of the human plasma fraction is also presented.

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19Dengue virus (DENV) causes the most prevalent and rapidly spreading arboviral 20 disease of humans. It enters human cells by receptor-mediated endocytosis. Numerous 21 cell surface proteins have been proposed as DENV entry factors. Among these, the 99 Regulation of lipid metabolism influences significantly the efficiency of DENV infection 100 and replication in vitro e in vivo [25-28]. Sequence stretches on DENV E and capsid 101 proteins resemble receptor binding motifs of ApoE [29]. ApoE is present in many 102 lipoproteins and interacts with several receptors of the low-density lipoprotein receptor 103 (LDLR) family [30]. Bovine lactoferrin, partially inhibits DENV and Japanese encephalitis 104 infection presumably competing for binding to the LDLR [31,32]. Collectively, these 105 results have suggested that LDLR may be involved on DENV entry to target cells. Yet, 106 conclusive evidences of the participation of LDLR on DENV attachment and/or entry 107 have not been obtained. 108 The LDLR family comprises at least 13 separate cell receptors that mediate lipoprotein 109 internalization, although some also play a role in the regulation of cellular physiology 110 and intracellular signaling [33]. In the context of the early events of the DENV infectious 111 cycle we decided to focus our interest on a different member of the family, the LRP1 112 receptor. This protein plays a role not only in lipoprotein endocytosis but also in the 7 113 internalization of protease:serpin complexes and, particularly of activated alpha-2-114 macroglobulin (α2M*):protease complexes [34]. We have found that proteins 115 participating in these processes are significantly enriched within the plasma DENV 116 interactome [35,36]. Also we have shown that α2M*, a ligand of LRP1, directly binds 117 DENV virions and favors DENV infection [37]. 118