AIM: Sambih getih leaves (SGL) contain flavonoids acting as an antioxidant and antihyperlipidemia. The purpose of this study is to determine the antioxidant activity and antihyperlipidemia effect of Ethanol Extract of Sambang Getih Leaves (EESGL).
METHODS: EESGL was tested for antioxidant activity in vitro and in vivo. Antihyperlipidemia test of SGL is done by looking at blood lipid parameters, that are total cholesterol, triglyceride, low-density lipoprotein (LDL), and high-density lipoprotein (HDL). 2,2-diphenyl-1-picrylhydrazyl (DPPH) of SGL and atorvastatin showed that Inhibition Concentration (IC50) values are 24.15 and 86.75 ppm. In vivo test was divided into six groups: Normal, negative, positive (atorvastatin), low dose 487.5, medium dose 975, and high dose 1950 mg/kg dose groups.
RESULTS: Measurements of Malondialdehyde (MDA) plasma levels of mice on that groups on 21st day were 1.91 ± 0.51; 5.86 ± 0.57; 2.18 ± 0.55; 4.33 ± 0.16; 3.08 ± 0.06; and 1.82 ± 0.19 nmol/mL. Measurements of Superoxide Dismutase (SOD) activity for each group were 193.29 ± 3.36; 89.41 ± 2.84; 192.46 ± 4.25; 175.53 ± 1.71; 191.53 ± 3.34; and 192.83 ± 2.88 U/mL. Results of total cholesterol level of positive (atorvastatin), low, medium, and high dose group were 62.16%, 40.50%, 48.55%, and 59.52%. The decrease in triglyceride levels was 48.94%, 27.78%, 34.27%, and 54.02%. The decrease in LDL levels was 82.77%, 57.43%, 65.18%, and 77.67%. Increased HDL levels were 20.24%, 17.94%, 17.08%, and 17.84%.
CONCLUSION: Based on the results obtained, it can be concluded that the EESGL have antioxidant activity and antihyperlipidemia but is not as good as atorvastatin.
