Highlights d The majority of disseminating cells of colorectal cancer are Lgr5 À d Lgr5 À cancer cells are the main seeds of colorectal cancer metastatic lesions d Long-term metastatic growth from Lgr5 À cells requires appearance of Lgr5 + cells d Lgr5 À metastases have the intrinsic capacity to re-establish the cellular hierarchy

SummaryAdipocytes, once considered simple lipid-storing cells, are rapidly emerging as complex cells with many biologically diverse functions. A powerful high-throughput method for analyzing single cells is flow cytometry. Several groups have attempted to analyze and sort freshly isolated adipocytes; however, using an adipocyte-specific reporter mouse, we demonstrate that these studies fail to detect the majority of white adipocytes. We define critical settings required for adipocyte flow cytometry and provide a rigid strategy for analyzing and sorting white and brown adipocyte populations. The applicability of our protocol is shown by sorting mouse adipocytes based on size or UCP1 expression and demonstrating that a subset of human adipocytes lacks the β2-adrenergic receptor, particularly in the insulin-resistant state. In conclusion, the present study confers key technological insights for analyzing and sorting mature adipocytes, opening up numerous downstream research applications.

Indian Centre for Space Physics has taken a novel strategy to study low energy cosmic rays and astrophysical X-ray sources which involve very light weight payloads up to about five kilograms on board a single or multiple balloons which are usually used for meteorological purposes. The mission duration could be anywhere from 3-12 hours. Our strategy provides extreme flexibility in mission preparation and its operation using a very economical budget. There are several limitations but our innovative approach has been able to extract significant amount of scientific data out of these missions. So far, over one hundred missions have been completed by us to near space and a wealth of data has been collected. The payloads are recovered and are used again. Scientific data is stored on board computer and the atmospheric data or payload location is sent to ground in real time. Since each mission is different, we present here the general strategy for a typical payload and provide some results we obtained in some of these missions.Keywords X-ray detectors and instrumentation · Secondary cosmic ray · X-ray sources · Meteorological balloon borne mission PACS 94.20.wq · 94.05.Sd · 95.55.-n · 95.55.Ka · 96.60.Q IntroductionStudy of the Universe in X-rays is a very important regime of astronomical observations. Since the atmosphere surrounding the earth obscures X-ray, we need to reach