The investigation was carried out for the isolation and characterization of the compounds from heart wood of root and root bark of Muntingia calabura. We have isolated six compounds, three from each extract and were identified as flavonoids. The bactericidal activity of these compounds found significant against tested bacterial strains. Among the tested compounds, 8-methoxy, 3ʹ,5ʹ7ʹ-trihydroxyflavone and 3,5,7-trihydroxyflavone (Galangin) showed paramount activity against MRSA. The results are compared with known standards gentamycin sulphate and cefixime.