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This study deals with the dairy wastewater treatment using laboratory scale vertical flow (VF) constructed wetlands with the <i>Canna indica</i> plantation over wetland beds due to phytoremediation capabilities. Three laboratory scale VF CWs (CW-A, CW-B and CW-C) each with an area of 0.135 m2 filled with gravel (CW-A: 20 mm; CW-B:10 mm gravel) and sand (CW-C) receiving 0.04 m3 d-1 dairy wastewater were operated for the wastewater purification. Each unit was operated at three hydraulic retention times (HRTs) i.e. 12 h, 24 h and 48 h for assessing its effect on wastewater purification . Among all units, removal rates fluctuated as: total suspended solids (TSS): 64.2–74.5%; biochemical oxygen demand (BOD): 45.3 – 63.1%; ammonium nitrogen (NH4–N): 29.6 – 56.5% and phosphate phosphorous (PO4–P): 20.5 – 57.8% at different HRTs. Increase in HRT showed better removal of pollutants in all CWs. Moreover, maximum removal of pollutants excluding TSS and NH4-N was achieved in CW-B at 48 h HRT. CW-B with similar HRT provided maximum removal of PO4-P (57.8%), BOD (63.1%) and chemical oxygen demand (COD): 67.4%. Increase in the size of filter media, from sand (0.25 mm) to 20 mm gravel resulted in higher removal of NH4 -N from wastewater.

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