Lichen, a symbiotic form of fungi and algae has been known for its metabolites, but, much of the metabolites remain unexplored. It's excellent biomedical potential with active substances are being applied for medicinal purposes for the past few years. By considering the active compounds, the present study aims at reporting the anti-microbial and anti-oxidant activities of less explored lichen, Ramalina inflata. Lichen sample was collected from Kolli hills of Eastern Ghats in the range of above 1000MSL. Further, it was subjected to extraction (Soxhlet) using five solvents and purification of bioactive metabolites. The result indicated the compounds that were present in the different extracts were found to be salazinic acid, divaricatic acid and some triterpenes. Antimicrobial activity of methanolic extract showed a high zone of inhibition against Klebsiella pneumoniae (13.01±0.12 mm). Petroleum ether showed the best inhibition against Staphylococcus aureus with 10.17±0.07 mm and against Candida krusei with an inhibition of 8.47±0.08 mm. Antioxidant assays of Phenolics, DPPH, Ferric reducing power assay, CUPRAC, and Phosphomolybdenum assay were done for various crude extracts. The result observed that benzene extract showed a high content of total phenolics, which was 30.790±0.903 μg/ml whereas, its percentage of inhibition in DPPH assay was 12.987%. The petroleum extract of R. inflata showed a moderate activity in ferric ion reducing power assay and molybdenum reducing assay and a very low cupric ion reducing capability. Methanol extract showed a high cupric ion reducing power with an optical density of 0.829±0.036.