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On the basis of the results bioengineered cell seeded scaffolds were found to be better than non-cell seeded scaffolds for the repair of abdominal wall defects in rats.

For CFU-F assay, 1×106 bone marrow nucleated cells per 60-mm dish were seeded and cultured at 37°C in 5% CO 2 for 12days with media change on every 2days, followed by crystal violet staining. The numbers of CFU-Fs were counted under a microscope. Quantification of osteoblasts (CFU-OB assay) For CFU-OB assay, 1×106 bone marrow nucleated cells per 60-mm dish were seeded and cultured for 21days in