In this study, root, leaf and fruit flavonoids of Tribulus terrestris L. are co mpared. Aqueous-ethanolic extracts of collected plant material were examined to practice flavonoid detection, isolation and identificat ion by 2-dimensional paper chromatography, thin layer ch ro matography and available references. Voucher specimen was prepared for reference as herbariu m voucher. Results showed chrysin was just identified in fruit while root and leaf had not any chrysin. Also flavone C and C-/O-glycosides were not found in leaf and fruit whereas root of the species had flavonoid sulphates and aglycones in addition to flavone C and C-/O-glycosides.