A comprehensive systematic investigation on diagnostic petiole characters of eighteen species of Tephrosia, from family Fabaceae, tribe Milleteae. It habits the fringes often forests, and abandoned, and wastelands. A total of nine diagnostic polymorphic characters were observed, photographed, assessed,scoredand coded as Data matrix for quantitative statistical treatment. The 9 characters codes include PSY, POL, PVS, PPT, and PTE. others include PTA, PTO, PTC, and PTB. Principal Components Analysis (PC) and Cluster Analysis were carried out. Four PC were identified and Cluster analysis indicates twelve hierarchies.