SYNOPSISThe relationships of the morphology and properties of a series of segmented polyurethaneurea ( P U U ) were studied in film form by using SEM and tensile stress-strain data. The polyether-based and polyester-based PUU block copolymers were synthesized using poly (propylene oxide )-ethylene oxide polyether or poly (butylene adipate) polyester with different molecular weights, 4,4-methylene bis ( phenyl isocyanate), and diethyltoluene diamine. The extent of phase separation increased with increasing molecular weight of the soft segment. The polyether-based PUU showed better phase separation than did polyesterbased PUU with the same molecular weight soft segment. The polyether-ester-based PUU showed the morphology of both polyether-based and polyester-based PUU but with larger domain size and interface.
SUMMARY
Maize is an important crop worldwide, as well as a valuable model with vast genetic diversity. Accurate genome and annotation information for a wide range of inbred lines would provide valuable resources for crop improvement and pan‐genome characterization. In this study, we generated a high‐quality de novo genome assembly (contig N50 of 15.43 Mb) of the Chinese elite inbred line RP125 using Nanopore long‐read sequencing and Hi‐C scaffolding, which yield highly contiguous, chromosome‐length scaffolds. Global comparison of the RP125 genome with those of B73, W22, and Mo17 revealed a large number of structural variations. To create new germplasm for maize research and crop improvement, we carried out an EMS mutagenesis screen on RP125. In total, we obtained 5818 independent M2 families, with 946 mutants showing heritable phenotypes. Taking advantage of the high‐quality RP125 genome, we successfully cloned 10 mutants from the EMS library, including the novel kernel mutant qk1 (quekou: “missing a small part” in Chinese), which exhibited partial loss of endosperm and a starch accumulation defect. QK1 encodes a predicted metal tolerance protein, which is specifically required for Fe transport. Increased accumulation of Fe and reactive oxygen species as well as ferroptosis‐like cell death were detected in qk1 endosperm. Our study provides the community with a high‐quality genome sequence and a large collection of mutant germplasm.
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