RESUMO Primeiro relato do Tomato severe rugose virus infectando plantas de pimentão no BrasilEste trabalho relata a primeira ocorrência do Tomato severe rugose virus (ToSRV) em plantas de pimentão (Capsicum annum) no Brasil. O vírus foi detectado em plantas coletadas nos municípios de Paranapanema e Piraju, São Paulo. O ToSRV é um begomovírus típico, descrito originalmente em plantas de tomateiro (Lycopersicon esculentum) e até o presente não havia sido detectado em pimentão.

In 2004 lettuce plants showing mosaic symptoms collected in São Manuel, SP were analyzed by electron microscopy, and particles with 730 nm typically from potyvirus were observed. After biological purification by monolesionals on Chenopodium quinoa, this isolate was sap inoculated on a host range assay. The virus infected C. quinoa and C. amaranticolor, causing local lesions and systemic mosaic. The virus did not induce symptoms on pea (Pisum sativum), but induced mosaic on the leaves of some lettuce cultivars such as Trocadero, White Boston, Regina, Verônica, Lucy Brown, Rafaela, Tainá, Vera and Laurel. The lettuce cultivar Gizele was tolerant to the virus. The coat protein gene was sequenced, and 97% aminoacids identity was observed with Bidens mosaic virus -BiMV (GenBank accession number AY960151). Differently of others previously BiMV isolates described, this isolate from lettuce was not able to infect Bidens pilosa, Helianthus annuus, Nicotiana tabacum TNN and N. glutinosa. The natural occurrence of BiMV infecting lettuce and causing mosaic symptoms similar to LMV, also the susceptibility of many lettuce cultivars to BiMV, could be an alert to the correct diagnosis of the viruses infecting this plant .

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Virus to the genus Begomovirus transmitted by whiteflies Bemisia tab ac i G., a nd considered one of the most importa nt fitossanitary problems for several cultures. Pepper plants were collected in eight the regions of São Paulo State, the extraction of total DNA and PCR were submitted with universal and degenerate primers wa s u sed to amplify partial sequences of the begomovírus coat protein gene. The data indicated the presence of begomovírus in five collected regions.

The complete nucleotide (nt) sequence of Bidens mosaic virus (BiMV) isolate SP01 was determined and shown to consist of 9,557 nt. Since it shared highest identities in the nt sequence of the whole genome (66-73 %) and in the aa sequence of the polyprotein (60-76 %) with viruses of the potato virus Y subgroup, it was compared with them genetically and biologically. Phylogenetic analysis showed that the closest relative of BiMV is sunflower chlorotic mottle virus, from which it, however, differed significantly in various respects. These results indicate that BiMV should represent a distinct species in the genus Potyvirus.