Anopheles funestus and Anopheles gambiae are malaria vector mosquitoes.Knowing their resting behavior is important for implementing control methods. The aim of this study was to investigate the resting behaviour of the two malaria mosquitoes. The study was conducted in Kilombero River Valley and mosquitoes were collected using indoor and outdoor traps from 2012-2015. Poisson mixed models were used to quantify the impact of environment variables on resting behaviour. A log ratio rate between the type of trap and its interaction with environmental variables was used to determine if there was a change over time in the resting behaviour. A total of 4696 mosquitoes were resting indoors of which 57% were A. funestus and 43% were A. gambiae. Similarly, a total of 12,028 mosquitoes were resting outdoor of which 13% were A. funestus and 87% were A. gambiae. Temperature was significant and affected the resting behaviour of A. funestus. Humidity, saturation deficit and temperature were significant variables influencing the resting behaviour of A. gambiae. A. funestus was resting indoor while A. gambiae was resting outdoor over time generally. The findings of this study on the effects of environmental variables and the variations in the resting behaviour of A. gambiae and A. funestus could be used as a guide to implementing appropriate intervention measures such as indoor residential spraying (IRS), insecticide treated nets (ITNs) and mosquito repellents.