This investigation was carried out from October 2011-June 2012 to determine the prevalence of Staphylococcus in milk and rate of contamination at farm and milk distribution tank in Alage Agricultural Technical Vocational Educational Training College. Milk samples (row) were cultured on sheep blood agar and incubated. The plates were examined for gross colony morphology, pigmentation and haemolytic characteristics at 24-48 h then presumptive colonies of Staphylococcus aureus was selected and sub cultured on nutrient agar and incubated. Then, bacteria were identified according to its Gram reaction, morphology and the catalase test, tube coagulase test (4 h), haemolysis, pigment production (golden yellow), mannitol and maltose fermentation were used. A total of 170 milk sample of cow were examined for bacteriological status of S. aureus, an overall 28.2% prevalence of Staphylococcus was found. From this, 21.2% was directly from the farm and 35.3% were from milk distributing site to consumers. The total prevalence of Staphylococcus varied among the sample taking site. The prevalence of S. aureus from distributing raw milk site (35.3%) was significantly higher than the prevalence of S. aureus from samples taken directly from the farm (21.2%). From this the study it is considered that Staphylococcosis a potential hazard for the public and contamination rate is high in distribution site which needs improvement of the hygienic status of the personnel's at distribution site.
This study was carried out from November 2007 to May 2008 to determine the prevalence and distribution of pulmonary lesions in different lobes in Jimma municipality abattoir. Cross sectional study by gross examination of cattle lung was done in the abattoir to determine pulmonary lesions. From 384 lungs examined, 91.7% was found to have one or more lesions. Hydatidosis (108, 28.1%), emphysema (115, 29.9%), atelectasis (72, 18.8%), congestion (191, 49.7%), abscess (21, 5.5%), and lung parasite (1, 0.3%) were found during the study period. Body condition association with the lesions were studied and pulmonary abscess and pulmonary emphysema has significant effect (p<0.05) on poor body conditioned animals. Animals with fat body condition were significantly affected (p<0.05) by pulmonary congestion and atelectasis. Lung parasite and hydatidosis found having no significant association with the animal body condition. In conclusion lung is a highly condemned organ due to different pulmonary lesions occurring which in turn results a significant economic loss.
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