Antimicrobial resistance gained by the microorganisms is a global threat, which leads to economic loss as there will be treatment failure, loss of life, discovery of newer and better antimicrobials of any infections as there is need for discovery of newer and better antimicrobials. A total of forty five samples were collected from clinical wound cases in and around Parbhani Veterinary Hospitals. The total of 39 Gram negative and 17 Gram positive isolates includes Pseudomonas (24), E. coli (15) and Staphylococcus (17) spp, were confirmed by biochemical characterization. Susceptibility of isolates to seven antibiotics was tested using disc diffusion method according to the guidelines defined by Clinical Laboratory Standard Institute. Pseudomonas Isolates showing resistance to antibiotic for Ceftazidime, Levofloxacin were for E.coli isolates showing resistance to antibiotics Erythromycin, Tetracycline and For Staphylococcus isolates Penicillin and Enrofloxacin were resistance.