Background: Rhodamine B is a component of xenobiotic substance metabolized by cytochrome P450 in the body to produce free radicals, which affects the activity of Superoxidase Dismutase (SOD), thereby, leading to oxidative stress, injury, increase in cell apoptosis and brainstem. This study aims to determine the effect of Rhodamine B on BAX and BCL-2 in the cerebellum and brainstem tissue of Rattus norvegicus.Design and Methods: The True Experimental Design was used to carry out a post-test examination on the control group of twenty-eight Wistar female Rattus norvegicus mice between the ages of 10-12 weeks. Then, samples were categorized into 4 groups in body weight doses of 4.5 mg/200g, 9 mg/200g, and 18 mg/200g. They were administrated with Rhodamine B personde for 36 days. Results: The results showed that Rhodamine B had a direct and indirect effect on BAX and BCL-2 expressions, respectively, in the cerebellum tissue and wistar strain of Rattus norvegicus. In addition, the positive path coefficient of BAX expression has a positive effect on BCL-2. This means that an increase in BAX has a direct impact on decreasing BCL-2 expression in cerebellum tissue and brainstem of Rattus norvegicus wistar strain along with an increased dose of Rhodamine B. Conclusions: In conclusion, Rhodamine B tends to increase BAX expression which directly decreases BCL-2 in Cerebellum tissue and Brainstem in Rattus norvegicus along with increasing doses.

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The high maternal mortality rate or MMR in Indonesia is an important issue that needs to be handled seriously. AKI is a measure of maternal health success and is a barometer of health services in a country, if the rate is still high, it means that health services in the country are categorized not good (Adriansz, 2007). So one of the efforts that need to get attention in reducing AKI is through improvement of health service quality of pregnant and maternity mother especially through antenatal care. The purpose of this study to determine the perception of pregnant women on antenatal care (ANC) in the Bolorejo village, Kauman District, Tulungagung Regency. This research is a qualitative research with cross sectional research design. Variable in this research is pregnant woman's perception on antenatal care service with population counted 11 responden, using technique of non probability sampling saturated (census), where research tool used is structured interview guidance, research location in Bolorejo village, Kauman district, Tulungagung regency. Time of study in July 2017. Presentation of data in the form of pie chart. From the results of the study obtained the results of pregnant women's perception on antenatal care service in the village of Bolorejo District Kauman Tulungagung district based on the care of ANC 10T that has been given to pregnant women has not been fully achieved. This is indicated from the data of the majority of pregnant women have received antenatal care 8T (80%) While a small proportion of pregnant women have not received antenatal care 2T (20%) which includes TT immunization and laboratory examination related to pregnancy (PP test, Hb, Protein urine, Urine reduction). Pregnant women who do not get TT immunization that there is 1 pregnant women due to pregnant women check into the village midwife in late third trimester of pregnancy and approaching delivery. As for pregnant women who do not get laboratory tests related to pregnancy (PP test, Hb, urine protein, urine reduction) that is as many as 3 pregnant women because the pregnant women did not want to be examined for fear of injections and no signs of danger pregnancy accompanying pregnant women during pregnancy (seen from the characteristics of respondents based on maternal reproductive age in low risk categories, based on parity also low risk). For pregnant women who are afraid of injections have actually been motivated by health cadres and village midwives but pregnant women are still not pleased to do laboratory examination (Based on the perception of pregnant women to public services and services ANC indicates that the services provided by health personnel are in conformity with standards) So that an unacceptable ANC examination of the standard (10T) was caused by a low awareness of pregnant women themselves. Keywords: Perception, Pregnant Women, Antenatal Care

Rhodamine B is a textile dye compounds containing chlorine (Cl-), alkylating (CH3-CH3), Poly Aromatic Hydrocarbons (PAH) which activate the enzyme cytochrome P-450 as well as the structure of quinone which is very redox that leads to the formation of Reactive Oxygen Species (ROS). ROS increases induce apoptosis of the intrinsic pathway. The imbalance ratio between BAX and BCL-2 stimulates apoptosis in Hippocampus tissue. “The selected design was “the post test only control group” using twenty-eight Wistar female Rattus norvegicus mouse age of 10-12 weeks. There was a significant difference (p-value <0.05) of total BCL-2 expression between the control group to the treatment group. Correlation coefficient of 0.945 indicates that the level of the relationship/ correlation is very strong category. Increasing doses of Rhodamine B was given, accompanied by the decrease in the expression of BCL-2. Correlation coefficient of -0.731 indicates that the level of the relationship/ correlation belongs strong category. It is concluded that Rhodamin B has been verified as capable to increase the expression of BAX and to reduce the expression of BCL-2 in hippocampus tissue on Rattus norvegicus.