The synthesis of amoxicillin derivate has been done by reacting amoxicillin with 4t-butylbenzoil chloride. The products were characterized by organoleptic test, melting point determination, TLC, UV/Vis Spectrophotometry, AND FTIR Spectrophotometry. Further, the antibiotics activity test of amoxicillin derrivate against Salmonella typhi were tested by using diffusion method. This product have been characteristic such as powder, light yellow, and bite bitter. Melting point of product was 132 °C. Rf value of product was 0.86. pH value of product amoxicillin derivate was 7.7. The results of UV wavelength scanning showed that product derivate of amoxicillin compounds had  peak at 286 nm. amoxicillin derivate had peaks in 3271 cm-1 (-NH amina), 2962 cm-1 (-CH aromatik), 1633 cm-1 (-C=O keton), dan other. Further, amoxicillin derivate was unable to clear zone formed around disk paper. N-4t-Butylbenzoyl was can’t be used for the treatment of thypoid fever.

Penelitian ini bertujuan untuk mengetahui pengaruh variasi konsentrasi gliserin sebagai plasticizer terhadap sifat fisik sediaan paper soap ekstrak daun nangka (Artocarpus heterophyllus Lam.). Konsentrasi gliserin yang digunakan adalah 7,5% (F1), 15% (F2), dan 22,5% (F3). Sifat fisik yang diteliti meliputi organoleptis (warna, bau, bentuk, kelengketan), pH, tinggi busa, kadar air, dan waktu tercuci. Hasil penelitian menunjukkan bahwa perubahan konsentrasi gliserin memberikan pengaruh terhadap sifat fisik organoleptis bentuk, kadar air, dan waktu tercuci sediaan paper soap dan tidak mempengaruhi organoleptis warna, bau, kelengketan, pH, dan tinggi busa sediaan paper soap. Formula dengan konsentrasi gliserin 22,5% memberikan karakteristik fisik terbaik.

Amoxicillin derivate compounds from the reaction of amoxicillin with p-aminophenol are synthesized compounds by reacting amoxicillin and p-aminophenol. . Escherichia coli is a flora normal bacteria that can causing infectious diseases. This research aimed to determine the antibiotic activity of amoxicillin derivat compound (amoxicillin with p-aminophenol) on the growth of E. coli. This research was conducted using the disc diffusion method. amoxicillin derivat compound (amoxicillin with p-aminophenol) were characterized by organoleptic test, melting point determination, pH test determination, Rf value, and maximum wavelength. The results of the organoleptic test showed that amoxicillin derivates from amoxicillin and p-aminophenol reaction is crystal powder, dark brown and bit bitter. Melting point of amoxicillin derivates from amoxicillin and p-aminophenol reaction was 160ºC. pH test of amoxicillin derivates from amoxicillin and p-aminophenol reaction was 6,5. Rf value of amoxicillin derivates from amoxicillin and p-aminophenol reaction was 0,79. The results of UV wavelength scanning showed that amoxicillin derivates from amoxicillin and p-aminophenol reaction at 295 nm. Antibiotic activity showed by the clear zone form around the paper disk. The results showed that E. coli was resistant to pure amoxicillin. Amoxicillin derivat compound (amoxicillin with p-aminophenol) with concentrations of 100% and 12,5% have the largest inhibitory zones (6,33 mm) compared with concentrations of 50% and 25%, 5,33 and 6,00 mm respectively. Based on these results it can be concluded that derivat compound of amoxicillin (amoxicillin with p-aminophenol) has antibiotic activity against the growth of E. coli that has been resistant to pure amoxicillin.

Diabetes mellitus (DM) is a disorder of the chronic metabolic system due to insufficiency of insulin function. DM is classified into two main categories, type 1 and type 2. The purpose of this study was to separate proteins using the SDS-PAGE method to determine the potential protein profile as a biomarker contained in the blood of type 2 DM patients, so that the results of the analysis can be used as an indicator of early detection of type 2 DM. This research is a true experimental research. The samples of this study were the blood of 20 patients with type 2 DM in Sukorejo Bangsalsari Village and the blood of 5 patients who non DM in Sukorejo Bangsalsari Village. sampling technique was randomized controlled trial, after the blood collection process, blood serum was then prepared at the Molecular Biology Laboratory, FMIPA UNEJ, then protein analysis was performed using the 1D-gel electrophoresis (SDS-PAGE) method. The results of protein characterization using SDS-PAGE analysis with blood serum of type 2 DM patients and serum of healthy people (non-patients) as negative controls, obtained protein bands that are less specific to potential targets, because the results of running are still not good, so it is not possible to know the biomarker protein profile in the blood of patients with type 2 DM in Sukorejo Bangsalsari Village, and the results of this analysis still cannot be used as an indicator for early detection of type 2 diabetes mellitus. Analysis of potential proteins profile as biomarkers using SDS-PAGE analysis has revealed three bands which had molecular weights of 28, 45 and 235 kDa.Keywords: Diabetes mellitus, Biomarker, SDS-PAGE

Diabetes mellitus (DM) is a disorder of the chronic metabolic system due to insufficiency of insulin function. DM is classified into two main categories, type 1 and type 2. The purpose of this study was to separate proteins using the SDS-PAGE method to determine the potential protein profile as a biomarker contained in the blood of type 2 DM patients, so that the results of the analysis can be used as an indicator of early detection of type 2 DM. This research is a true experimental research. The samples of this study were the blood of 20 patients with type 2 DM in Sukorejo Bangsalsari Village and the blood of 5 patients who non  DM in Sukorejo Bangsalsari Village. sampling technique was randomized controlled trial, after the blood collection process, blood serum was then prepared at the Molecular Biology Laboratory, FMIPA UNEJ, then protein analysis was performed using the 1D-gel electrophoresis (SDS-PAGE) method. The results of protein characterization using SDS-PAGE analysis with blood serum of type 2 DM patients and serum of healthy people (non-patients) as negative controls, obtained protein bands that are less specific to potential targets, because the results of running are still not good, so it is not possible to know the biomarker protein profile in the blood of patients with type 2 DM in Sukorejo Bangsalsari Village, and the results of this analysis still cannot be used as an indicator for early detection of type 2 diabetes mellitus. Analysis of potential proteins profile as biomarkers using SDS-PAGE analysis has revealed three bands which had molecular weights of 28, 45 and 235 kDa. Keywords: Diabetes mellitus, Biomarker, SDS-PAGE