Background: Premenstrual Syndrome (PMS) is a common psychosomatic disorder which affects 30%-50% of women in child bearing age. Symptoms’ have devastating effect which starts early in life. So, the study was conducted to estimate the prevalence of premenstrual syndrome (PMS) and its association with menstrual profile among female students of colleges situated in Ujjain city.Methods: Observational follow-up study was conducted at 4 colleges selected by convenience sampling located in Ujjain city. PMS and menstrual profile related data from 250 girls (18-25years) who consented to participate were collected using a modified version of daily record of severity of problems for atleast 2 consecutive menstrual cycles. Diagnosis of PMS was made using ACOG criteria. Data analysis was done using SPSS version 16.0.Results: The prevalence of PMS was 39.6% and was found to be negatively associated with any history of mothers having menstrual problems.Conclusions: Frequency of PMS is relatively common in young girls and has negative association with any history of mothers having menstrual problems. Measures should be adopted so that mothers can condition their daughters about premenstrual symptoms which will influence the quality of life of young females.