Mycoplasma gallisepticum (M. gallisepticum) is a bacterium that causes chronic respiratory disease (CRD) and infectious sinusitis (IS) in chickens and turkeys. Therefore, rapid and immediate diagnosis or regular detection of Mycoplasma may be of great help to early detection. 120 chicken layers, Within Karbala city, were carried out during their laying period on breeding flocks. The study proposed a promising method for isolation of M. gallisepticum, 120 tracheal swabs and blood samples from chickens in different dairy farms were used to analyze M. gallisepticum utility of PCR and culture. Compared with ELISA anti-IgG M. gallisepticum, the clinical specificity of PCR detection is 89.66%, the sensitivity is 86.36%, and the kappa coefficient is 0.817. Compared with the culture method, the specificity is 100%, the specificity is 45%, and the kappa coefficient is 0.543. Demonstrate the method's effectiveness and applicability as a standard method for mycoplasmas field diagnosis.

This Study was conducted in animal house in Faculty of veterinary medicine of university of kufa. This study was designated to assess the neuropathy of Organophosphorus pesticide (Methomyl) after long-term administration of low dose in Rock pigeons. The Clinical signs, gross lesions and histopathological assessment of nervous tissue were described. A total of 20 pigeons, 3months old and about 500g average body weight pigeons were used in this study. The pigeons were equally divided in to tow groups. The dose was calculated based on study at close pilot at 0.02 ml and gradually lose the dose down to the toxic non-lethal dose. The toxic dose was 0.01 ml. One group was daily administered via oral gavages with 0.01 ml of methomyl; for 21 days where as the other group were leave control without administration. Organophosphorus pesticide treated groups exhibited both muscarinic and nicotinic signs of toxicity.