Objective: the objective of this work was to investigate the phytochemical groups and to detect phenolic acids in L. camara leaves using highperformance thin-layer chromatography (HPTLC). Methods: Leaves of L. camara were macerated thrice a week in petroleum ether, then in ethanol. Powdered leaves and ethanolic extract were subjected to phytochemical investigation. Acidic hydrolysis were performed on the extract, and then fractionated by petroleum ether, chloroform, ethyl acetate, and n-butanol. The chloroform and ethyl acetate fractions were analyzed by HPTLC for their phenolic acid contents. Result: L. camara leaves devoid of alkaloids. Three different phenolic acids were detected in the leaf extract. Conclusion: Gallic acid, caffeic acid and p-coumaric acid were detected in L. camara leaves
Abstract:
Borago officinalis is highly interesting amongst nutritional and medical source relate to its high composition of some useful phytochemical compound. It is great plants with bright blue star-shaped flowers present in most world regions and usually known as borage. The Borago phytochemical analysis showed the presence of alkaloids, tannins, flavonoids, phenolic acids, essential oil, vitamins and others. Borage is cultivated all over the world and used in traditional medicine as a demulcent, diuretic, emollient, tonic, expectorant, for the treatment of coughs, inflammation and swelling, and other diseases. In herbal medicine, Borage seed oil (BSO) has been utilized for many progressive illnesses as a treatment agent. The BSO compounds include p-simen-8-L, ?-caryophyllene, sesquiterpenes, monoterpenes, alpha-linoleic, gammalinoleic and linoleic acid. BSO is famed to be the richest vegetable origin of gamma (?)-linoleic acid (GLA). Borage officinalis possess different pharmacological properties including antinociceptive, antioxidant, radioprotective, memory improving, hepatoprotective, antinflammatory, and analgesic effects.
Burdock ( Arctium lappa), is among the most popular plants in traditional medicine and it is associated with several biological effects. Literature survey revealed the presence of phenylpropanoid compounds .The most widespread are hydroxycinnamic acids ( mainly caffeic acid and chlorogenic acid) and lignans (mainly arctiin and arctigenin). This work will confirm the presence of these compounds in Arctium lappa, cultivated in Iraq, in both root and leaf samples. The dried plant samples were extracted by soxhlet with 80% methanol then separated the main constituents by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Identification of the isolated compounds was carried out by UV, IR, and compared with reference standards using TLC, HPLC and HPTLC.
Keywords: Actium lappa, hydroxycinnamic acid, Lignan, Phytochemical.
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