Two studies, using 128 crossbred, multiparous beef cows grazing native winter and spring rangeland, were conducted to determine effects of protein supplementation on nutrient status and subsequent calf and reproductive performance. Postparturient cows (body condition score [BC] = 3.9) were fed 1.82 kg of one of two supplements (54% CP at 490 g of CP/d) per cow on alternate days. Supplements were formulated to contain 50% (245/490 g of CP) ruminally undegradable (UD) CP (RU50) or 25% (119/490 g of CP) UD CP (RU25) and were fed until breeding (BC = 4.2). Late-calving cows (Study 1) were individually fed supplement while grazing native range. Early-calving cows (Study 2) were group-fed supplement on alternate days while grazing native range and were daily fed approximately 5.4 kg of medium-quality grass hay (10.5% CP). Late-calving cows had similar (P > .22) BW losses postpartum, whereas early-calving cows fed RU50 lost 39 kg less (P < .01) BW than did cows fed RU25. Cows that received RU25 returned to estrus sooner (P < .02), in the late-calving study, than cows fed RU50 (47 vs 56 d, respectively). The percentage of cows serviced in the first 21 d of breeding (96 and 100% for late- and early-calving studies, respectively) and pregnancy rates (88 and 86% for late- and early-calving studies, respectively) did not differ regardless of postpartum protein supplement. When adequate CP was provided in the diet for optimum function of the rumen, addition of ruminally undegraded CP decreased weight loss of mature, postpartum beef cows grazing native range.(ABSTRACT TRUNCATED AT 250 WORDS)