The contents of total flavonoid(TF) and total phenolic acid(TPA) in ethanol extracts from bamboo leaves of 21 species of Phyllostachys were determined, and the scavenging effects of ethanol extracts from bamboo leaves on DPPH and ABTS were also studied. The results showed that the ethanol extracts from bamboo leaves of 20 species of Phyllostachys contained rich flavonoid and phenolic acid, total content of which were respectively 8.963 mg/g～28.0614 mg/g and 12.3767 mg/g～31.8915 mg/g. Through the studies of antioxidant activities, it indicated that ethanol extracts of different bamboo species all had potent antioxidant activities, which was positively correlated with total flavonoids and phenolic acid. This paper provided a scientific basis for the research of active constituent and the exploitation of bamboo leaves from different species.