In Dengue Hemorrhagic Fever (DHF) is a health problem and the number of cases is increasing every year, including in East Java. Blitar City is one of the cities in East Java which is endemic dengue. The Incident Rate (IR) in 2015 which is 70,28 per 100.000 population over the target area that is 21 per 100.000 population. One effort that can be done to reduce the number of dengue cases is increasing activeness jumantik in the prevention of dengue. Jumantik task in the prevention of dengue fever include CHD (Flick Periodic Monitoring), larvasidasi and reporting results of CHD to the clinic regularly. The purpose of this study was to determine and analyze the relationship between training, motivation and availability of facilities with participation jumantik in Blitar City. This study used an analytic observational study with cross sectional study design. Respondents in this study are jumantik in Blitar City with a sample of 171 samples. The data used in this study are primary data and secondary data. Statistical test results obtained value of meaningful relationship training (p = 0,000), motivation (p=0,000) and the availability of facilities (p = 0,000).Conclusions of this research that thereare a relationship between the training, motivation and availability of facilities with participation jumantik in Blitar City. Suggestions for Blitar City Health Department and clinics in Blitar City which should jumantik training ongoing each year and monitor the results of CHD regularly to anticipate the incidence of DHF.Keywords: Blitar City, Jumantik, Participation

Zingiberaceae is a family of plant that has been widely used to treat various diseases and as an element of spice in cooking. In this paper, the potential of the extract from the steam distillation residue of Zingiberaceae leaves as antibacterial and biofilm degradation agent was studied and determined against Streptococcus mutans. Five different species of Zingiberaceae, which consisted of Curcuma longa, Curcuma zeodoaria, Curcuma aeruginosa, Zingiber officinale, Zingiber cassumunar were taken for samples and their distillation residues were extracted by soxhlation using 3 different solvents namely n-hexane, ethyl acetate, and methanol. The antibacterial and biofilm degradation activity of the assay from each of the samples was determined by the microdilution technique. Among the 15 Zingiberaceae leaves distillation residue extracts, five are categorically active against Streptococcus mutans with their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values being the same with that of chloramphenicol, 15.63 μg/mL. All extracts were found to degrade the biofilm. The methanol extract of C. zeodoaria leaves was found to have the highest antibacterial activity with MIC and MIB vaues of 15.63 ppm and the best to degrade the biofilm with inhibitory concentration 50% (IC50) of 15.33 ppm. The antibacterial and biofilm degradation activities of extracts are not related to the phenolic content and it was suggested that terpenoid such as (-)-zingiberene may have been the active component.

Dengue Haemorrhagic Fever is an infectious disease caused by the dengue virus which is transmitted mainly through the bite of the Aedes aegypti mosquito. Currently, larvicides are an important strategy in dengue vector control. Larvisides are applied to mosquito breeding sites to kill larvae. This study aims to determine the effectiveness of citronella essential oil as a larvicide for Aedes aegypti. An experimental design with a randomized post-test only control group design was applied in this study using the first to fourth instar larvae of Aedes aegypti as the test material. The concentrations of essential oils applied were 10 ppm, 50 ppm, 100 ppm, 500 ppm and control with 4 replications for 3 different days. A total of 25 Ae aegypti larvae were used in each treatment and observed for 24 hours. Data analysis was performed using univariate and bivariate (probit test and Kruskal Wallis test). The results showed that all concentrations in the treatment group had significant differences with the control group with p < 0.05. This laboratory test also revealed that citronella essential oil was effective to kill the fourth instar larvae of Aedes aegypti with an LC50 of 1.553 mg/L. Citronella essential oil was effective in killing 50% of IVth instar larvae (LT50) within 3.6 minutes. The results of the chemical composition analysis of citronella essential oil using GC-MS showed citronellal, 2,6-octadien-1-ol, 3,7-dimethyl, and citronellol as the most abundant compositions. Citronella essential oil has potential as an effective botanical pesticide to control  Aedes aegypti larvae. Abstrak Demam Berdarah Dengue adalah salah satu penyakit menular yang disebabkan virus dengue yang ditularkan dari seseorang ke orang lain melalui nyamuk Aedes aegypti. Saat ini, larvasida merupakan salah satu strategi penting dalam pengendalian vektor dengue. Tujuan dari penelitian ini adalah untuk melihat efektivitas minyak atsiri sereh wangi sebagai larvasida Aedes aegypti. Metode yang digunakan berupa eksperimental design dengan rancangan randomized post test only control group design dengan menggunakan larva Ae. aegypti instar I sampai IV sebagai material uji. Konsentrasi minyak atsiri yang digunakan yaitu 10 ppm, 50 ppm, 100 ppm, 500 ppm dan kontrol dengan  empat kali pengulangan selama tiga hari berbeda. Masing-masing perlakuan menggunakan 25 larva dan dilakukan pengamatan selama 24 jam. Analisis data dilakukan secara univariat dan bivariat (uji probit dan uji kruskal wallis). Hasil penelitian menunjukkan bahwa semua konsentrasi pada kelompok perlakuan memiliki perbedaan secara bermakna dengan kelompok kontrol p<0,05. Minyak atsiri sereh wangi pada semua konsentrasi perlakuan efektif dalam membunuh larva Ae. aegypti.  Konsentrasi minyak atsiri sereh wangi yang paling baik membunuh 50% (LC50) larva Ae. aegypti instar IV sebesar 1,553 mg/L. Minyak atsiri sereh wangi mampu membunuh larva Ae. aegypti instar IV sebanyak 50% (LT50) pada waktu 3,616 menit. Komponen utama minyak sereh atsiri sereh wangi yang teridentifikasi  dengan GC-MS adalah  citronellal, 2,6-octadien-1-ol, 3,7-dimetil, dan citronellol. Minyak atsiri sereh wangi dapat berpotensi sebagai larvasida botani yang efektif untuk mengontrol nyamuk Ae. aegypti