The effecto fd ifferentp rotectants and the impact of the initial cell densityo n the viability of Lactococcus lactis Sr. 3.54 subjected to freeze-thawing and freezedrying was studied. Several additivesw ere tested as protective agents against freezing and drying injuries. Maximumv iability of the cells after freeze-thawing was obtained with sucrose and skim milk mixtures as protective agents (78% viability).
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