* autor a quien debe ser dirigida la correspondenciaRecibido Abr. 25, 2016; Aceptado Jun. 7, 2016; Versión final Jul. 6, 2016, Publicado Dic. 2016 Resumen El objetivo de este trabajo fue aumentar polifenoles en el sustrato del fruto de algarrobillo mediante fermentación en estado sólido (FEZ) con Rhizopus sp. (CMPUJ H041). Las condiciones del sustrato fueron 125 °C y 150 kPa durante 20 min y 180 µm. Posteriormente, se establecieron dos muestras (con y sin (NH4)2SO4) de 150 g, aplicando un diseño estadístico completamente al azar con tres repeticiones. A estas muestras se les adicionó la solución nutriente y el inoculo con concentración de esporas 4x10 6 esporas/mL, y luego se procedió a la FES. Los polifenoles fueron determinados colorimétricamente usando el método de Folin-Ciocalteu. El contenido de polifenoles se incrementó durante la FES y los resultados fueron estadísticamente significativos (p < 0.05) obteniéndose 7.324 mg EAG/g de peso seco de muestra (sin (NH4)2SO4) y 11.74 mg EAG/g de peso seco (con (NH4)2SO4). La FES incrementó los compuestos polifenólicos del sustrato, lo que es importante para posibles usos en fuentes de consumo. Palabras clave: Samanea saman; polifenol; Rhizopus sp.; fermentación en estado sólidoIncreased Polyphenols in Algarrobillo substrate (Samanea saman (Jacq.) Merr.) through Solid State Fermentation with Rhizopus sp (CMPUJ H041) AbstractThe objective of this study was to increase polyphenols in the fruit of algarrobillo substrate through of the solid state fermentation (SSF) with Rhizopus sp. (CMPUJ H041). Substrate conditions were 125 °C and 150 kPa during 20 min. and 180 µm. Subsequently, two samples of 150 g were drawn (with and without (NH4) 2SO4) and a completely randomized statistical design with three replications was applied. To these samples a nutrient solution and inoculum with spore concentration 4x10 6 spores/mL were added, to then proceed with the SSF. Polyphenols were determined calorimetrically using the Folin-Ciocalteu method. The polyphenol content increased and the results were statistically significant (p < 0.05) obtaining 7.324 mg EAG/g dry weight (without (NH4)2SO4) to 11.74 mg EAG/g dry weight (with (NH4)2SO4). SSF polyphenolic compounds enhanced total substrate, which is important for possible uses in consumption sources.
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