Background: There are urgent calls to dismantle systemic racism and discrimination in pharmacy education and practice. It is recommended that educational programs critically review learning materials for occurrences of stigmatization that may reinforce population biases and stereotypes. Previous studies, as well as premises from race and queer theories, suggest that written cases that do not appropriately include or acknowledge patient diversity may promote a "hidden curriculum" that may foster implicit biases in student healthcare professionals. Objectives:The purpose of this study was to explore the existence of a "hidden curriculum" within case-based learning materials by determining the extent to which underrepresented populations were represented in case descriptions and how representation occurred within groups.Methods: This was a qualitative content analysis of written patient cases (n = 76) used throughout the problem-based learning curriculum at the College of Pharmacy, Dalhousie University (Halifax, Nova Scotia, Canada). Proportions were calculated for each variable identified to represent the categories of interest (race, gender, sexual orientation, relationship status, and presence of disabilities). Quantified data were analyzed and themes were identified to represent each category assessed.Results: Data across all categories were mostly "undefined." The most defined variables within each category were: white (race, 17.1%), female (gender, 53.9%), heterosexual (sexual orientation, 35.2%), married (relationship status, 29.6%), and wheelchair (disability, 1.3%). Defined variables were representative of dominant cultural groups with little representation of underrepresented populations. Themes identified were undifferentiated (race), binary (gender), heteronormative (sexual orientation), traditional (relationship status), and absent (disabilities). Conclusion:Findings support the notion that there may be a "hidden curriculum" reinforcing biases and stereotypes due to the undefined nature of the cases. Educators should prioritize inclusion of diversity within cases as part of a coordinated plan, in order to ensure representation is appropriate and well-distributed across the curriculum.