Background: Gingival crevicular fluid (GCF) is a biological fluid derived from the gingival sulcus and can be elevated in the inflammatory state of periodontal tissue, such as gingivitis. In previous studies, the number of GCF could also increase after panoramic radiographic exposure. Increase in GCF due to panoramic radiography is a sign of cell damage. Objective: To analyze the effects of panoramic radiography on the volume of GCF in wistar rats with gingivitis. Method: This type of research was true experimental with post test only and control group design. The sampling technique used was simple random sampling. A total of 25 wistar rats were classified into two control groups without exposure (state without gingivitis and gingivitis) and three groups treatment of exposure (state of gingivitis with 1 time, 2 times, and 3 times the exposure). GCF sampling using filter paper was carried out 10 minutes after panoramic radiographic exposure. The filter paper was stained by 2% ninhydrin solution, after that the GCF volume was calculated. Results: There was a significant difference in the number of GCF (p<0.05) in the group without gingivitis and exposure compared to all other groups, the gingivitis group without exposure compared to the gingivitis group with 3 times exposure, and the gingivitis group with 1 time exposure compared to the gingivitis group 3 times with exposure. Conclusion: Panoramic radiography can cause an increase in the volume of GCF in wistar rats with gingivitis. Keywords: GCF, gingivitis, panoramic radiography

Background: Panoramic radiography is one of the most commonly used radiographic diagnostic tools. Panoramic radiography exposure can increase the GCF flow because of increasing vascular permeability.GCF flow can also increase in periodontitis conditions. Objective: The purpose of this study was to analyze the effects of 1, 2, and 3 times exposure of panoramic radiography to the volume of GCF in wistar rats with periodontitis. Method: This research was true experimental with post test onlycontrol group design. Thesampling technique used simple random samplingthat obtained 25 wistar rats comprising of 5 wistars from each group in which the GCF samples of each group was collected from. Results: The average number of GCF inhealthy rats groupthat was not exposed to panoramic radiographywas 0.114 µL, the periodontitis rats group that was not exposed to panoramic radiography was 0.246 µL, the periodontitis rats group that was exposed1 time was 0.286 µL, the group of periodontitis rats exposed 2 times was 0.294 µL, and periodontitis group of rats exposed 3 times was 0.374 µL. The results of the One-Way Anova and Post Hoc Bonferroni tests stated that there is a significant increase in the volume of GCF between the control group and all other treatment groups, as well as between the non-exposed periodontitis group and the periodontitis group with 3 times exposure. Conclusion: Panoramic radiography can causeincrease the volume of GCF in wistar rats with periodontitis. Keywords: Gingival Crevicular Fluid,panoramic, periodontitis, wistar rats

Background: Periodontitis is a periodontal disease that can cause an increase in Reactive Oxygen Species (ROS). The increase in ROS concentration will cause oxidative stress which has a role in DNA damage. In the case of periodontitis, panoramic radiography plays an important role in showing generalized horizontal bone loss. Radiation on panoramic radiography may cause DNA damage. DNA damage that occurs due to periodontitis or panoramic radiographic radiation exposure is characterized by the formation of micronuclei in gingival epithelial cells. Objective: To determine the effect of panoramic radiography on the number of micronuclei in Wistar rats with periodontitis. Method: This study was true experimental with post-test only and control group design. This study used 15 male Wistar rats which divided into 5 groups. Result: The average number of micronuclei in the normal group and 1 time exposures was 4 compared to the periodontitis group without exposure was 1.67, periodontitis and 1 time exposures was 8, periodontitis and 2 times the exposure was 15.67, and in the periodontitis and 3 times the exposure was 42.67. Result of One-Way Annova test and Post Hoc Bonferroni test indicated that signigicant changes in the number of micronuclei was seen between the normal group with 1 time exposure to the periodontitis group with 2 times and 3 times exposure and int the periodontitis group without exposure to the periodontitis group with 2 times and 3 times exposure. Conclusion: Panoramic radiograph X ray radiation and periodontitis can cause changes in the number of micronuclei in wistar rats. Keywords: , Micronucleus, Panoramic Radiography, Periodontitis.