In this study, an accurate, simple, economical and precise Reversed-Phase High Pressure Liquid Chromatography (RP-HPLC) method was developed for the simultaneous estimation of Ozenoxacin and Benzoic Acid in a pharmaceutical cream formulation, according to the International Conference on Harmonisation (ICH) guidelines. Chromatographic separation was achieved by gradient elution, on RP-HPLC Instrument, equipped with column C8 (150 mm × 4.6 mm, 5 μm particle size) using Ultra Violet (UV) detector at 235 nm wavelength, by using Mobile Phase A: triethylamine, trifloroacetic acid and water (1:1:1000) and Mobile Phase B: methanol and Diluent: water, acetonitrile and triethylamine (500:500:1), at flow rate 0.8 mL min−1; injection volume of 20 μL; column oven temperature 45 °C and sample temperature: 25 °C; Run time: 15 min. All the validation parameters were within the acceptance criteria, as per ICH requirements, for Ozenoxacin and Benzoic acid. Consequently, this method has found to be validated, simple, rapid and successfully applicable, to the simultaneous estimation of Ozenoxacin and Benzoic acid by RP-HPLC, for routine analytical testing in quality control, with a run time of 15 min and for future research studies. Forced degradation of Ozenoxacin cream 1% w/w formulation was performed and found that validated method has stability indicating potential that needs to be further studied.

The current investigation was carried out to find available fluoride concentration in groundwater in Kurnool District, Andhra Pradesh where the major source for drinking is ground water. Around 141 samples are collected from bore wells and hand pumps of selected villages in Kurnool district by using Grid map. The samples were analyzed for selected Physico-chemical parameters. The fluoride ion content was found out in the range of 0.2 to 3.6 mg/l with mean 1.1 mg/l. Human risk assessment was carried out in terms of Hazard quotient. The Average Hazard Quotient observed for children were 1.13, for males were 1.04, and for females it is 0.92. The observed HQ values are above 1 which may prone to risk, particularly for children due to their low body weight. Compared to BIS water quality standards, the remaining parameters are also within the permissible limit except nitrate ion. The elevated nitrate level in groundwater was due to excess utilization of nitrogen based fertilizers.