Pluchea lanceolata is an important medicinal plant of Asteraceae family known for its anti-arthritic and anti-inflammatory activity. A protocol was established for micropropagation of P. lanceolata using nodal explants. Nodal explants were inoculated onto Murashige and Skoog (1962) - MS medium supple–mented with 6-benzylaminopurine (BAP), kinetin (Kin), thidiazuron (TDZ) and 2iP (2-isopentenyladenine) at various concentrations (0.0, 0.5, 1.0, 1.5 and 2.0 mg·dm-3). The highest multiplication rate was obtained for nodal explants cultured on MS medium, supplemented with 0.5 mg·dm-3 thidiazuron (TDZ). In vitro raised shoots were successfully rooted on ½ mineral salt concentration of MS medium supplemented with 1.0 mg dm-3 IBA.