The present work has been undertaken to study the in vitro response in widely used cultivars WesternHB-55, TAG 24 and SB11 of peanut having desired agronomic characteristics. The mature embryo derived leaflet explants of all three peanut genotypes were found morphogenic in vitro. When comparing the effects of TDZ and BA with respect to caulogenic response, it can be inferred that TDZ is a more potent inducer of caulogenesis in vitro than BA. All genotypes responded equally to different concentrations of BA. WesternHB-55 responded better than others which results in high proliferation rate in vitro. The present study emphasizes on the usage of WesternHB-55 germplasm through adventitious organogenesis pathway in transformation studies (biolistic gun approach), for the production of proteins, vaccines enzymes etc.