The present study concerns the isolation and characterization of potential probiotic bacteria isolated from indigenously fermented cereal-based products commonly produced by tribal people of the Aravali hills region of India and the documentation of their unexplored probiotic attributes. The isolated strains were evaluated for probiotic attributes, such as bile salt and acid tolerance, lysozyme and phenol tolerance, antagonistic and antifungal activity, cell autoaggregation, cell-surface hydrophobicity, simulated gastric and pancreatic digestion, antioxidative potential, bile salt hydrolase activity, and H2O2 production. The safety of isolates was assessed by antibiotic sensitivity, hemolytic activity, DNase activity, and biogenic amine production assays, while technological properties, such as fermenting ability, amylolytic activity, and EPS production, were also evaluated. A total of 70 LAB isolates were screened initially, and 6 strains showed good potential as probiotic candidates in in vitro assessments. The efficient strains were identified using phenotyping and biochemical characterization, which results were further confirmed and recognized at the strain level using phylogenetic analysis and 16S rDNA sequencing. The current study has shown that Lactiplantibacillus plantarum KMUDR7 isolated from “Makka ki Raab” has excellent probiotic attributes and could be a potential probiotic for product preparation. However, other strains, Lactobacillus delbrueckii subsp. bulgaricus KMUDR1 and Lacticaseibacillus rhamnosus KMUDR9, showed good properties, while KMUDR14, -17, and -20 also have comparable probiotic attributes.

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In the present study, cow and camel raw milk showed positive clot on boiling (COB) test after 6 h and 14 h respectively. Pasteurization enhanced the shelf life of cow milk twice without any substantial increase in the shelf life of camel milk. Both raw and pasteurized camel milk had better shelf life at ambient (25°C) as well as refrigerated (7°C) temperatures than that of cow milk. Development of acidity was much slower in camel milk as compared to cow milk. Further, camel milk was found to be less prone to lipolysis as compared to cow milk. FFA content in COB positive camel and cow milk was 0.7 meq/L for 14 h storage and 1.9 meq/lit for 6 h storage only. Camel and cow raw milk showed positive alcohol test at 6 h and 4 h of storage at ambient (25°C) temperature respectively. Pasteurization and refrigeration enhanced the shelf life of camel and cow milk.

Ayurveda is not only a science of medicine, but also a theory of living life with awareness. Addiction is Habitual psychological or physiologic dependence on a substance or practice that is beyond voluntary control. It is a chronic brain disease that causes compulsive substance use despite harmful consequences. Without a clear understanding of the mental and physical effects of marijuana, its use on ouryouth, our families, and our society, we will never understand the ramifications it will have on thelives of our younger generation, the impact on their future, and its costs to our society. Globally, it is estimated that some 243 million people corresponding to some 5.2 per cent of the world population aged 15-64 had used an illicit drug. The Ayurveda has ancient science which has stores of herbal, mineral or herbomineral medication for prevention and cure of disease person including Addiction and Withdrawal of drugs abused. The padansik kram to withdrawal of any substance has suggested in Ayurveda which is unique method to tapper up the abused drugs or replacement of that abused drugs. [1] Recreational therapy, Psychological Counseling is also suggested only in Alcoholism. [2,3] In this study management of cannabis addiction as per Ayurveda has discussed. 2. AIMS AND OBJECTIVE 1.To evaluate, elaborate and discussed the Cannabis addiction. 2.To evaluate, elaborate and discussed the solution of Cannabis addiction as per Ayurveda. 3. MATERIAL AND METHOD Material related to Cannabis addiction andit "solution is collected from Ayurvedic text and text book of modern medicine respectively. The available commentaries of Ayurvedic

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