Hervani D, Efendi D, Suhartanto MR, Purwoko BS. 2018. The preservation of somatic embryos of papaya derived from papaya lateral shoots after being stored in cryopreservation to maintain plant genetic information in the future. Biodiversitas 19: 724729. Germplasm storage of papaya is very important because this plant easily adapts to genetic changes due to environmental conditions and open system pollination, so it is necessary to retain the current genetics resources in order to conserve the genetic information. The storage of the vegetative part of the plant with cryopreservation is expected to retain the plant's genetic information in the future. Cryopreservation is the method for germplasm storage using liquid nitrogen at temperature of -196oC This experiment aimed to obtain the growth ability of papaya lateral shoots to produce somatic embryos after being stored by cryopreservation. The experiment was designed in factorial by Completely Randomized Design with two factors.The first factor was the immersion time duration in PVS2 as cryoprotectant solution with 5 treatments of immersion duration of 0, 10, 20, 30, and 40 minutes. Second factor was culture medium for cultivated the lateral shoot which was added with plant growth regulators such as BA (benzyl adenine) and NAA (naphthalene acetic acid) at levels of 0, 1, 2, 3, and 4 mg l-1, respectively. The results showed that the immersion of lateral shoot in cryoprotectants for 10 and 20 minutes gave the better plantlet survival rate after discharge from liquid nitrogen, while the treatment of culture media had not been significant difference.
Sugar palm is a bioenergy plant with great potential in food security, biopolymers, biofuel, and herbal medicine. Sugar palm seed germination is indicated by the appearance of cotyledon petiole, where there are several different positions. Morphological characteristics of sugar palm seeds at various positions of cotyledon petiole are not currently available. This research aimed to study the morphology characters of sugar palm seedlings with different cotyledon petiole positions. The research applied Experiment in Completely Random Design with four treatments, each repeated three times. The four treatments are the cotyledon petiole position, namely P1: cotyledon petiole in right-center, P2: cotyledon petiole in left-center, P3: cotyledon petiole in left-bottom, and P4: cotyledon petiole in right-bottom. The results showed that morphologically, there was no significant difference in the sugar palm seedling growth with each cotyledon petiole position [256 DAS]. Range of seed height 47,63-57,50 cm, range number of leaf midribs 2-3,33, range of primary root length 13,73-21,76 cm and, range of secondary root length 28,60-44,26 cm.
Sumatera barat adalah salah satu penyumbang komoditi buah alpukat dari Indonesia. Buah alpukat (persea Americana mill), masyarakat kurang mengetahui bahwa bukan hanya daging buah alpukat yang berkasiat bagi kesehatan, tetapi biji buah alpukat juga berperan karena kandungan gizi dan antioksidan serta serat. Kandungan antioksidan yang tinggi dan persentase kandungan pati yang tinggi membuat biji alpukat memungkinkan menjadikannya pangan fungsional dalam bentuk tepung biji alpukat. Beberapa tahun ini telah dilakukan penelitian pemanfaatan biji buah alpukat (Persea americana Mill) dalam menghasilkan produk, baik dalam bidang teknologi pangan (makanan dan minuman), kimia, farmokologi dan seni rupa. Artikel ini dibuat dengan menggunakan metode Systematic Literature Review (SLR), untuk mengetahui diverfikasi produk olahan biji alpukat sudah ada, dan merupakan teknologi yang bisa diterapkan ditengah masyarakat.
Indonesia mempunyai beragam sumberdaya pangan sehat yang sangat berpotensi untuk dikembangkan, salah satunya ialah Hanjeli. Diketahui bahwa tanaman serealia ini memiliki bentuk fenotipik yang beragam serta adaptif untuk tumbuh pada berbagai kondisi lingkungan. Beragamnya karakteristik antar genotipe hanjeli akan berpengaruh terhadap mutu biji baik secara kualitas maupun kuantitas. Cara membedakan karakter khas dari masing-masing genotipe hanjeli, diperlukan pengamatan dan pengukuran secara detail salah satunya pada organ perkembangbiakan yakni biji. Penelitian ini bertujuan untuk mengevaluasi mutu fisik biji hanjeli lokal Sumatera Barat sebagai langkah untuk mendapatkan genotipe yang potensial dari segi kuantitas maupun kualitas. Enam aksesi hanjeli yang digunakan meliputi PTA-1, KKD-3, PH-4, BTA-2, GT-2, dan TJR-2. Percobaan ini menggunakan Rancangan Acak Kelompok dengan 3 ulangan sehingga didapatkan 18 satuan percobaan. Disimpulkan berdasarkan dimensi aksialnya (panjang, lebar dan tebal biji) menunjukkan bahwa aksesi dengan kode BTA-2 dan KKD-3 memiliki bentuk dan ukuran biji terbesar dan aksesi dengan kode GT-2 sebagai aksesi dengan bentuk dan ukuran biji terkecil. Pada pengamatan tingkat kekerasan biji, ketebalan epicarp, bobot 100 biji pecah kulit serta rendemen biji pecah kulit aksesi dengan kode PH-4 menunjukkan sebagai genotipe terbaik.
The conversion of fertile land into industrial areas and construction development has contributed to the decline of rice production in Indonesia. Therefore, it is necessary to expand the rice fields to the sub-optimal land, i.e. lands with high levels of Fe and Al and low soil pH. One of the solutions that can potentially solve the problem is choosing varieties of rice that can adapt to the suboptimal land environment; the rice varieties can be obtained by estimating rice genetic parameters and selecting the optimal planting methods. One method that can potentially improve rice yield is “System of Rice Intensification”, or SRI method. Our study was conducted in the rice fields on suboptimal land at Pasar Ambacang, Kuranji District, Padang, Sumatra, from December 2019 to April 2020. The aims of the experiment are to determine the genetic parameters and responses of the three rice varieties to suboptimal land using the SRI method. A complete randomized design with four replications was used to set up the experiment using three varieties of rice, i.e. “Batang Piaman”, “Bujang Marantau”, and “IPB3S”. Based on the plant growth measurement and analysis of the genetic parameters, we found that the plant growth characters, particularly height and panicles length, had high heritability and genetic progression values.
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