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Tuna fish image classification is an important part to sort out the type and quality of the tuna based upon the shape. The image of tuna should have good segmentation results before entering the classification stage. It has uneven lighting and complex texture resulting in inappropriate segmentation. This research proposed method of automatic determination seeded random walker in the watershed region for tuna image segmentation. Random walker is a noise-resistant segmentation method that requires two types of seeds defined by the user, the seed pixels for background and seed pixels for the object. We evaluated the proposed method on 30 images of tuna using relative foreground area error (RAE), misclassification error (ME), and modified Hausdroff distances (MHD) evaluation methods with values of 4.38%, 1.34% and 1.11%, respectively. This suggests that the seeded random walker method is more effective than exiting methods for tuna image segmentation.