to be glycosylated by a high-mannose-type oligosaccharide. Northern hybridization analysis of poly(A)+ mRNA from etiolated maize coleoptiles revealed a single mRNA species of approximately the same size as the cDNA isolated. The axrl gene is differentially expressed in RNA isolated from different organs of a maize plant, showing the highest level of expression in maize styles and ears.

Manufacturing of cellular products for therapeutic purposes like stem cell or cancer therapy requires equipment with specific characteristics not always addressed by conventional technologies. A new integrated cell processing device is presented that can handle all current technical requirements for manufacturing cellular products by automation of the complete process in a GMP‐compliant single‐use tubing set. Its capabilities are exemplified in the presented study by successful processing of adult stem cells, natural killer cells, and several cell lines. Multiple cell processing workflows can be automated in a functionally closed environment: from cell separation through cell culture to formulation of the final product.

The sexuality-inducing glycoprotein of Volvox carteri f. nagariensis was purified from supernatants of disintegrated sperm packets of the male strain IPS-22 and separated by reverse-phase HPLC into several isoforms which differ in the degree of 0-glycosylation. Total chemical deglycosylation with trifluoromethanesulphonic acid yields the biologically inactive core protein of 22.5 kDa. This core protein possesses three putative binding sites for Nglycans which are clustered in the middle of the polypeptide chain. The N-glycosidically bound oligosaccharides were obtained by glycopeptidase F digestion and were shown by a combination of exoglycosidase digestion, gaschromatographic sugar analysis and two-dimensional HPLC separation to possess the following definite structures:Two of the three N-glycosidic binding sites carry one B and one D glycan. The A and C glycans are shared by the third N-glycosylation site. The 0-glycosidic sugars, which make up 50% of the total carbohydrate, are short (up to three sugar residues) chains composed of Ara, Gal and Xyl and are exclusively bound to Thr residues.

The synthesis of the sex-inducing glycoprotein of Volvox curferi was investigated. A highly specific antiserum directed against pheromone deglycosylated with trifluoromethane sulphonic acid was used to detect inducer protein in maturing cultures of the male strain IPS-22. Immunoblot analysis showed that inducer synthesis is restricted to a very late phase of sperm development, a few hours before the sperm cells die. The pheromone is co-translationally glycosylatcd to yield a well-defined pattern of isoinducers.

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