The main cause of premature aging of the skin is the presence of free radicals which are created by UV rays. The use of antioxidants is a promising option for overcoming these problems. Duwet (Syzygium cumini) is a natural source of antioxidants. A peel-off mask is a cosmetic product which forms an easy to peel-off layer on the skin’s surface. This study aimed to determine the physicochemical characteristics, antioxidant activity, irritation effect, and stability of duwet leaf extract peel-off mask preparations. Duwet leaf extract was macerated by a methanol solvent and formulated into the peel-off masks with a concentration of 1%, 3%, and 5%. The results showed that the preparation produced good characteristics with a drying time of fewer than 30 minutes and showed significant antioxidant activity with the highest inhibition in the preparation with 3% duwet leaf extract. This preparation also had no irritating effect on CAM and was stable during storage using freeze-thaw and real-time methods. It can be concluded that duwet leaf extract can be used as an active agent for anti-aging peel-off masks. Keywords: duwet leaf extract, peel-off mask, physicochemical characteristics, stability test, antioxidant activity, irritation effect