Aegle marmelos is a medicinal herb that has a variety of biological constituents. “Catechins” are a class of phenolic compounds that have therapeutic value. The present study employs batch extraction with methanol as a solvent to extract the catechins from the pulp of Aegle marmelos fruit. Box–Behnken design of response surface methodology (RSM-BBD) is used to optimize the operational parameters impacting catechin extraction, such as solvent concentration, pH, and extraction time. Extraction of 96.5% of catechins was achieved at a methanol concentration of 80%, pH 6.24, and a soaking period of 44.7 hrs (desirability: 0.966). Additionally, MATLAB’s artificial neural network (ANN) was used to accurately estimate the extraction yield. The antimicrobial activity of the methanolic extract was tested against five different pathogens, including Streptococcus, Bacillus mega, Pseudomonas putida, Bacillus cerecus, and Staphylococcus aureus, using the agar diffusion method and the tube dilution method. Streptococcus, Bacillus cereus, and pseudomonas putida showed high activity.

In this study, the catalytic mechanism of cetrimonium bromide (CTAB), a cationic surfactant,by pure ZnO nanoparticles was investigated.Further observed ZnO nanoparticles synthesization by precipitation method in aqueous solution with sulfates of metallic precursors.Adding,XRD(X-Ray Diffraction) and SEM(Scanning Electron Microscope) were observed for the crystalline and structural analysis of synthesized ZnO nanoparticles.SEM shows obtained ZnO nanoparticles are of size 180.4 nm.For phase identification o f a crystalline material XRD was used and to observe chemical properties of ZnO nanoparticles,studied FTIR (Fourier Transmission of Infra-Red).The maximum percentage removal of CTAB was found to be 95% by varying different conditions by time,dosage,pH and temperature parameters.