Background and objectives: Oral hygiene is mandatory for patients with orthodontic appliances and prevention of microbial biofilms in the oral environment. One method for inhibiting the growth of microorganisms is commonly by use fluoridated dental material. The aim of this study was to evaluate releasing fluoride after addition into the orthodontic acrylic resin with 40% hydroxyethyl methacrylate, and amount of fluoride release from the orthodontic acrylic resin that was polymerized by Auto polymerization and IVOMAT polymerization. Materials and methods: Sixty disc-shaped samples measured 10 mm diameter and 1mm thickness of orthocryle were divided into two groups according to polymerization technics, thirty samples for Auto polymerization and thirty samples for polymerization by IVOMAT. Each of these groups was subdivided into three groups according to the concentration of NaF. Group A 0% NaF, Group B 10%NaF, and Group C 20%NaF. For testing fluoride release, samples stored for 28 days in deionized distilled water for testing measurements for days 1,4,7,14,21, and day 28. Precision ion meter was used to measure fluoride release. Repeated measure ANOVA was undertaken to compare fluoride release in each group. Paired sample t-test was utilized to differentiate between groups. Results: Significant difference was observed in the fluoride-releasing of both groups and within the subgroups (P≤0.05). The highest amount of fluoride release is present in 20% NaF followed by 10% NaF for Auto polymerized acrylic resin in the first day. The lowest fluoride release is presented in 10% on day 28 for IVOMAT polymerization. In general, the Auto polymerized acrylic resin had higher fluoride release than IVOMAT from all groups for 0%,10% and 20% NaF. Conclusion: Orthodontic acrylic resin has the ability to release fluoride more than 28 days for both types of polymerization technics in different concentrations.