Abstrak: Keong mas (Pomacea canaliculata Lamarck) merupakan hama potensial tanaman padi sawah yang berkembangbiak dengan sangat cepat sehingga sulit untuk menekan perkembangannya. Salah satu jenis tanaman yang dapat mengendalikan dan menekan populasi hama ini adalah pinang (Areca catechu L). Penelitian tentang pengaruh pemberian ekstrak pinang terhadap kesintasan telur keong mas, telah dilaksanakan di Laboratorium Biologi Undana Kupang. Tujuan penelitian ini adalah untuk mengetahui: 1) pengaruh pemberian ekstrak biji pinang terhadap kesintasan telur keong mas, (2) nilai LC50 ekstrak biji pinang terhadap mortalitas telur keong mas, (3) nilai LT50 ekstrak biji pinang terhadap mortalitas telur keong mas. Dalam penelitian ini digunakan rancangan acak lengkap dengan enam perlakuan yang terdiri dari tiga ulangan. Data penelitian dianalisis dengan analisis ragam dan analisis probit untuk menghitung LC50 dan LT50. Hasil penelitian menunjukkan bahwa (1) pemberian ekstrak pinang efektif dalam menghambat dan mematikan telur keong mas serta memberikan pengaruh nyata terhadap kesintasan telur keong mas. (2) nilai LC50 ekstrak pinang adalah pada konsentrasi 18.899% dengan garis regresi Y=10.56 + 1.98x dan (3) nilai LT50 ekstrak pinang yaitu pada 72,980 jam. Pemanfaatan ekstrak pinang sangat direkomendasikan bagi petani padi sawah yang ingin membasmi hama keong mas.Kata Kunci : A. catechu L., Telur P. canaliculata Lamarck, Kesintasan.Abstract: Golden snail (Pomacea canaliculata Lamarck) has been potential pest for rice plant which quickly develops, therefore it is difficult to stop its growth. One kind of plant which is able to stop the growth is areca (areca catechu L.). The influence of giving areca extract toward the development of golden snail’s eggs has already been done by the Biology Laboratory of Undana Kupang. The aim of the study is to know: 1) the influence of areca extract toward the development of golden snail’s eggs; 2) the LC50 value of areca extract on the mortality og golden snail’s eggs; 3) the LT50 value of areca extract on the mortality of golden snail’s eggs. Random technique was used with six sessions of treatment and three tests were implied. The analysis was done by classifying and also probility to count the value of LC50 and LT50. The result showed that (1) giving areca extract was effective to restrain and stop the development of golden snail’s eggs and giving obvious influence toward the development of the eggs. (2) the value of LC50 areca extract was on the concentration of 18,899% with the regression was Y=10.56 + 1.98x and (3) the value of LT50 areca extract was on 72.980 hours. Therefore, making of areca extract is highly recommended for farmers especially in the rice field who want to prevent the grouth of golden snail.Keywords : A. catechu L., P. canaliculata Lamarck egg, Survivorship.
Dafala village is one of the villages in NTT that uses lenglengan leaves as traditional medicine to treat diseases such as coughs, TBC, diarrhea and back pain.This present research aims to know the bioactive compounds and composition of the bioactive contained in Lenglengan (Leucas lavandulifolia Sm.) leaf extract, and too know the effectiveness of lenglengan leaf extract in inhibiting Staphyloccocus areus bacteria. We extracted leaves of Lenglengan by maceration method using ethanol. The extract was obtained and then tested in a qualitative way to determine the bioactive compounds are made by using reagents. While in the quantitative test to Determine of levels of flavonoids, phenols and tannins using UV-Vis spectrophotometry method, alkaloids and saponins using the gravimetric method. The Antibacterial test was carried out by disc paper method. Paper discs containing extract concentrations: 12.5%, 25%, 50%, 62.5% and 75%, positive control (gentamicin) and negative control (aquadest) were placed on MHA media containing S. aureus suspension, incubated at 37°C for 24 hours and then the diameter of the inhibition was observed. Qualititive test result show that the lenglengan leaf extract contains 5 bioactive compounds include alkaloids, flavonoids, saponins, tannins, and phenols. The composition of bioactive compounds in Lenglengan leaf extract: flavonoids 23.93%, saponins: 19.05%, alkaloids: 15.28%, tannins: 5.81%, and phenols: 2,335%. The atibacterial result reveal that lenglengan leaf extract was significantly affect in inhibiting Staphylococcus aureus. The higher concentration of extract lenglengan leaves used, the higher the inhibition zone produced. The highest level response inhibition showed from level of 12.5%: 11.3 mm, 25%: 12.4 mm, 50%: 13.43 mm, 62.5%: 14.43 mm and 75%: 15.58 mm, gentamicin: 17.4 mm aquades: 0 mm.
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