In this article we generalized results of sodium fluorescein and indocyanine green usage in removing of brain tumors and arteriovenous malformations. Basic principles of fluorescence, it’s sensitivity and specificity are thoroughly described. Drug’s dosages, their ways and exposition of administration are indicated. Special attention is payed at fluorophores’ use in removing of various brain lesions.Concerning fluorescence with indocyanine we reviewed «second window» effect, expanding its usage in neurooncology. In conclusion we described other perspective methods of fluorescence that are being actively researched but have not been implemented in clinical practice yet.