Purpose: To evaluate the effect of three natural materials (Ginger and honey mixture and rosemary oil) on remineralization and prevention of enamel white spot lesions measured by color change using vita easy shade. Material and methods: The study was carried out in two phases remineralization and prevention phase, sixty four anterior teeth were used for each phase. Teeth were prepared and randomized into four groups according to treatment material (n=8 samples). Group I: control group which receives no treatment. Group II: treated with 1.23% sodium fluoride gel. Group III: treated with ginger-honey mixture. Group IV: treated with rosemary oil. Treatment material was applied for 3 or 6 minutes. In phase (1) color was measured at baseline, after creation of white spot lesion, and after treatment. In phase (2) color was measured at baseline and after treatment. Data were obtained and the differences between groups were estimated by ANOVA test followed by Tukey's post hoc test. Results: for phase1:There was a statistically significant difference among different groups. The highest mean value of ΔE was for sodium fluoride gel group followed by ginger and honey group at 6 min. For phase2: group III had a better acid resistance effect followed by group II at 6 min. Conclusion: Natural materials can be used for remineralization and prevention of WSL as ginger and honey mixture enhance remineralization following sodium fluoride gel which was more effective. And for acid resistance phase, ginger and honey mixture was more effective.

Purpose: To evaluate the effect of Miswak and Grape seed extract (GSE) on enamel and dentine subjected to artificial acid challenge in-vitro. Materials and Methods: A total of thirty sound freshly extracted sound human permanent periodontally affected upper molars were used. 30 enamel specimens were cut from the buccal surface of the teeth and 30 dentin specimens were cut from the cervical root area. All specimens were polished to obtain a flat polished surface. Ethanolic miswak extract was prepared by percolation method. Solutions of 10% ethanolic miswak extract, 6.5% Grape seed extract and 0.05% sodium fluoride were prepared. Specimens were divided into two groups (n=30); enamel and dentin. Each group was further divided into three sub-groups (n=10) according to the material used. Group1; treated with 10% ethanolic miswak extract solution. Group2; treated with 6.5% GSE solution. Group3; treated with 0.05% sodium fluoride solution (positive control). Specimens were individually subjected to alternative treatment/pH cycling regimen for 7 days. Radiodensity (change in mineral content) for each enamel and dentine specimen was assessed before and after pH cycling using digital radiography. Data were statistically analyzed; the significance level was set at p ≤0.05. Results: Miswak and GSE showed a high percent gain in enamel and dentine radiodensity compared to fluoride. Conclusion: Miswak and GSE are beneficial to both enamel and dentin through increasing their resistance to acid attacks.