We evaluated the analytical performance of the Immulite 2000 immunoassay analyzer (Diagnostic Products Corporation, Los Angeles, USA) based on a new detection technology, electrochemical luminescence. The evaluated analytes were thyrotropin, triiodothyronine, free thyroxine, follitropin, lutropin, prolactin, cortisol, estradiol and progesterone. We tested the assay precision, linearity, recovery, and correlation with comparison methods for these analytes. For most assays, within-run and between-day imprecisions were less than 8% and 10%, respectively. The linearity and recovery were acceptable for all assays. The correlation between the Immulite 2000 assays and comparison methods showed satisfactory results.