Leptospires are difficult to stain with the usual bacterial stains; many different stains have been proposed, most of which are designed for staining of tissue sections. Silver stains that are available for smear preparations yield variable, unreliable
A technique for leptospiral lipase extraction which yielded a highly active, stable, and concentrated lipase preparation was developed. The chief characteristics of leptospiral lipase were determined and are summarized below. Leptospiral lipase was soluble in water and stable in both the dry state
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