No abstract

A liquid chromatographic method for the determination of allopurinolin tablets was collaboratively studied by 7 laboratories. The methoduses a C,8 reverse phase column, a 0.05M ammonium phosphate mobilephase, hypoxanthine as the internal standard, and photometric detectionat 254 nm. Collaborators were supplied with samples of 2 commercialtablets and 1 synthetic tablet powder. The mean recovery value of allopurinol from the synthetic tablet powder was 100.0%. The combinedmean coefficient of variation for all 3 types of sample analyzedwas less than 2%. The method has been adopted official first action.

A liquid chromatographic method for the determination of flucytosine in capsules was collaboratively studied by 7 laboratories. The method uses a C18 reverse phase column, water-methanol-acetic acid mobile phase containing 1-octanesulfonic acid sodium salt, p-aminobenzoic acid as internal standard, and photometric detection at 285 nm. The mean recovery value (±SD) of flucytosine from a synthetic formulation representing capsules was 99.2 ± 1.72% (CV = 1.73%). Composited samples of 250 and 500 mg commercial capsules gave assay values of (mean ± SD) 103.17 ± 2.21 and 99.29 ± 1.29% of declared, respectively. CV values were 2.15 and 1.30%. Reproducibility and repeatability CVs were 2.19 and 1.50%, respectively, for the 250 mg capsules, and 1.34 and 0.63%, respectively, for the 500 mg capsules. The method has been adopted official first action.

A liquid chromatographic method has been developed for determination of flucytosine in capsules. Flucytosine and p-aminobenzoic acid, the internal standard, are separated on a C18 reverse phase column using water-methanol-acetic acid mobile phase containing 1-octanesulfonic acid sodium salt. Compounds are detected photometrically at 285 nm. Mean assay results for 250 and 500 mg commercial capsules were 101.5% (n = 5) and 100.2% (n = 5) of declared, respectively. Mean recovery of flucytosine added to commercial capsules was 99.3 %.