Taking the extraction voltage, liquid-solid ratio and extraction time as the factors, and the yield of Ligustrum lucidum seed oil as the evaluation index, response surface methodology was used to explore the best technology of Ligustrum lucidum seed oil flash extraction method, and its antioxidant activity was analyzed. The results showed that the optimum extraction conditions were as follows: voltage 140 V, ratio of liquid to material 12:1, extraction time 150s, and the oil yield was 13.53%. The results showed that Ligustrum lucidum seed oil had certain scavenging ability to DPPH · free radical and hydroxyl radical, and its scavenging ability were positively correlated with its mass concentration. When the mass concentration was 2.5mg/ml, the DPPH · free radical scavenging rate was 32.25% and the hydroxyl radical scavenging rate was 43.83%.