Objective: To investigate the value of cooperative learning model in the nursing skills of practice nurses and standardized training for newly-recruited nurses. Methods: 130 practice nurses and 80 newly-recruited nurses from two Grade-three hospitals in Hunan Province were selected as objects of study, and randomly divided into two groups. The practice nurses and newly-recruited nurses in control group received traditional clinical nursing teaching and training, and those in intervention group receive clinical nursing teaching and training under cooperative learning model, the score, self-learning ability and patient satisfaction in the two groups were compared. Results: After 1 month of learning, the theoretical scores and skill scores of practice nurses and newlyrecruited nurses in intervention group were significantly higher than those in control group (P<0.05). The practice nurses and newly-recruited nurses in intervention group recognized that the training model improved their learning initiative, awareness of cooperation, ability to think, communication skills and comprehensive ability compared with those in control group (P<0.05). Conclusion: Cooperative learning model can improve the effectiveness of training for practice nurses and newly-recruited nurses, which not only promotes nurses' theoretical knowledge and nursing skills, but also trains the ability of cooperation, thinking and communication, so it is conducive to practice nurses and newly-recruited nurses in the new post.