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Construction and preliminary characterization of open tubular capillary column ion chromatography is described. Increasing the column temperature resulted in a dramatic increase of the column efficiency. Two different kinds of columns were developed and investigated for capillary ion chromatography. One was a 50µm i.d. fused silica capillary tubing, and another was a 50µm i.d. silica capillary coated with Latex particles (diameter: about 360nm). Both columns showed good separations of inorganic anions, although the capillary coated with Latex particles showed much larger retention than a bare silica capillary.

We demonstrate the possible application of the sandwich type surface-enhanced Raman scattering (SERS) immunoassay using antigen-antibody binding for detection of prostate-specific antigen (PSA) in cancer cells. In this sandwich type of SERS immunoassay, to capture antigens onto the immobilized layer of antibodies on the gold substrate we prepared the monolayer of gold nanoparticles on the APTMS-derivatized surface of a glass slide by using the SAM technique. This sandwich type of SERS immunoassay in which antigens on the substrate specifically capture antibodies on a Raman reporter (DSNB coated gold nanoparticles with R6G) could successfully detect PSA at low levels. A strong SERS spectrum of Raman reporter was observed only with a substrate in which PSA is present.