The search for rennet substitutes such as microbial rennet has increased fold due to increase in the demand for cheese products. Microbial rennet covers about one-third of the cheese consumption worldwide. Hence it is important to develop commercially viable and cost efficient method for purification of rennet from microbial sources. Hence the present work was focused on the production and purification of microbial rennet from Aspergillus candidus. The rennet was purified using a two step purification process involving solvent precipitation and chromatographic separation. The purity of the milk clotting enzyme was increased by 7.43 fold by solvent precipitation using equal mixture of 150% (v/v) ethanol-acetone. Then the enzyme was further purified using Diethylaminoethyl (DEAE) cellulose chromatography and 10.45 fold increase in enzyme activity was obtained after purification. The temperature of 35°C and substrate concentration of 0.25 mg/ml were found as optimum for maximum enzyme activity. The kinetics of the purified enzyme was studied and the Michaelis-Menten parameters such as rate constant (Km) and the maximum reaction rate (Vmax) were found as 0.059 mg/ml and 8.59 x 10-3 mmol/ml/sec respectively.

In the present work, Microspheres of Sumatriptan Succinate using PLGA, Ethyl cellulose and HPMC K4M as polymers were formulated to deliver Sumatriptan Succinate via oral route. The results of this investigation indicate that solvent evaporation method can be successfully employed to fabricate Sumatriptan Succinate microspheres. In this work an effort was made to formulate microsphere of Sumatriptan Succinate by using different polymers. Prepared formulations are evaluated for bulk density, tapped density, precent mucoadhesion, Percent compressibility, hausners ration, percentage yield, size and interaction study by Differential scanning calorimeter and in vitro drug release. Formulation which passed all the evaluation parameters was considered as best formulation of Sumatriptan Succinate. The present study conclusively that Sumatriptan Succinate microsphere could be prepared successfully and formulation F5 was shows satisfactory result.