A knowledge of the factors concerned in the growth of yeasts is of value for theoretical plant science and biochemistry, and provides information for practical application in the field of microbiological assays, the production of yeast for fodder and food, and the development of fermentative industrial processes. In this paper some data are presented on the vitamin requirements of 163 strains of yeasts belonging in 110 named species and varieties. Dosage response curves of a yeast which is deficient for 6 B vitamins and an illustration of the-application of such information to the assay of vitamins are given. No attempt is made here to review the literature in this field; some valuable references may be found in the citations given in our earlier papers (Burkholder, 1943;Burkholder and Moyer, 1943).Grateful acknowledgment is made to the following persons who supplied cultures for this work: L. J. Wickerham,

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