In this study, the effect of concentration of indole-3-butyric acid (IBA) (powder dip), cutting type as well as the time of taking cuttings on the rooting of softwood cuttings of cornelian cherry was examined. Four types of cuttings were used: basal cuttings, terminal cuttings, basal cuttings with 2-year-old wood and terminal cuttings with 2-year-old wood. The obtained results showed that IBA concentration, cutting type and time of collecting have significant effect on rooting. The best results were obtained using 1% IBA and cuttings collected in the second term (in mid-July) had a higher rooting percentage. Terminal cuttings treated with 1% IBA (powder dip) should be used for optimum results. In that case the rooting percentage was very high (over 90%) in both terms of cuttings collection. [Projekat Ministarstva nauke Republike Srbije, br. 43007: Istrazivanje klimatskih promena na zivotnu sredinu: pracenje uticaja, adaptacija i ublazavanje]
Several in vitro biotechnological techniques have been developed, all of which require a reliable protocol to produce a responsive callus mass. One of these techniques is callus fusion in vitro, which is reliable for the early detection of (in)-compatibility of scions and rootstocks. In this paper, the possibility to obtain friable callus tissues was explored by callus induction of adult tissues of Japanese flowering cherry trees from the group Sato zakura (Prunus serrulata 'Amanogawa', 'Kanzan' and 'Kiku-shidare-zakura') and two domestic cherry rootstocks -Prunus avium and Prunus 'Colt'. The explants used in the research were: leaf petiole, leaf base with a part of a petiole, part of lamina with a midvein and a stem with an axillary bud. Among three plant growth media (MS, SH and WP) that were used in this study, the MS proved to be the most favourable for the majority of taxa during the callus induction process. For the sweet cherry tree and the cultivars 'Kanzan' and 'Colt', the SH plant growth medium was also acceptable. The best results in callogenesis were obtained for the majority of taxons with auxin at the concentration 2 mgL . It is also possible to use 2.4-D at the same concentration as a substitute for the genotypes Prunus avium, Prunus 'Colt' and Prunus serrulata 'Kanzan', whereas IBA proved to be an inappropriate auxin for callus induction. The protocol described herein is proved to be efficient callus induction in a range of taxa of genus Prunus.
Amorpha fruticosa is in many countries, and also in Serbia, one of the most dangerous invasive species. The detection of mechanisms which inhibit the spreading of Amorpha population would be of great significance for finding the adequate measures of biological control. In this sense, first of all it is necessary, inter alia, to determine Amorpha ecological and coenological characteristics. The vegetation studies of wet sites in Belgrade were compared. The study results indicate that Amorpha is present in forest communities, from the lowest elevations at the timber line to the highest elevations, where the periods of underground and flood waters are the shortest, or where they are absent. Amorpha forms thick brushland on meadows with different water regimes and in forest plantations of different canopy closure. If it is regularly removed by felling, Amorpha does not endanger the structure and the floristic diversity of meadow communities. Amorpha is absent or rare in forest plantations of the densest canopy, as well as in the communities which belong, or once belonged, to the alliance Magnocaricion Br.-Bl
The study deals with the influence of growth regulators on the germination of Judas tree (Cercis siliquastrum L.) seed which is double dormant. We simultaneously tested seeds prepared in the conventional procedure: scarification + stratification and scarified seeds treated with phytohormones from the groups of gibberellins, auxin and cytokinins. The results indicate a positive effect of gibberellic acid (GA), as well as some combinations of this phytohormone with others. Recommendations for practice are to combine the conventional procedure with GA. The procedure may shorten the duration of stratification; the application of GA should follow stratification because the temperature of 4?C does not provide growth regulators activity. The study results can serve as the base for easier generative reproduction of this valuable woody ornamental species which could have a wide use in changed climate conditions. [Projekat Ministarstva nauke Republike Srbije, br. 43007: Studying climate change and its influence on the environment: impacts, adaptation and mitigation]
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